Identification of the transferrin receptor as a novel. This process requires a tight regulation of gene expression. Cd71 megakaryocyte myeloblast cd61 neutrophil cd15 cd16 stem cell and early progenitor cells cd3, cd271, cd117 stem cell, early and late progenitor cells cd34 basophil cd123 eosinophil cd44 platelets cd61 erythrocyte monocyte cd14 cd11b macrophage promonocyte mdc1 cd1c mdc2 cd141 myeloid dendritic cells myeloid progenitor cell cd33. Evaluation of cd71 expression in acute leukemia in. We have tried to evaluate the expression of cd71 in various types of acute leukemias. By engineering a known human mutation, linked to a myeloid disorder in patients with trisomy 21, we have created a model to further study trisomy 21associated hematopoietic disease.
Most of the known iga abmediated functions require an interaction with the iga receptors, four of which have been identified in humans. This crosssectional study included 37 bone marrow samples of acute leukemia cases diagnosed between october 2016 to april 2018. We estimated by quantitative flow cytometry fc the expression of cd, cd33, cd34 and cd117 antigens in cells from 64 patients with acute myeloid leukaemia aml and 22 normal bone marrows bms. Flow cytometry data was acquired on a beckmancoulter fc500 flow cytometer beckman coulter, inc. Flow cytometry evaluation of erythroid and myeloid. This study objected to assess the expression of cd71 in patients with acute leukemia and to address its prognostic significance and relations to clinicopathologic features. Biomarker expression patterns in human immune cells.
Early data from jak2 inhibitor clinical trials have called into question the capacity of these compounds to alter the natural history of jak2v617fmediated mpn. Pdf regulation of transferrin receptor in myeloid and. In contrast, in control mice lower cd71 expression was observed data not shown. Dendritic cells dc are the most effi tocompatibility complex mhc. Pdf prognostic value of transferrin receptor1 cd71. Pdf differential expression and function of iga receptors. Biomarker expression patterns in human immune cells colors represent different lineage pathways. Cd71 or transferrin receptor is expressed on the surface of erythroid lineage cells.
Review of the relevance of aberrant antigen expression by. Circular rna expression in human hematopoietic cells is. Cells were collected 36 to 48 hours after the second transfection and analyzed for cd71 surface expression. Dendritic cells dc represent a heterogeneous cell population, residing in most peripheral tissues, particularly at interfaces with the environment skin, mucosae, where they represent 12% of all cells. Upper gating strategy for hscsmpps, cmps, gmps, and meps. Transferrin receptor1 expression was analyzed on the bone marrow blasts by flow cytometry at time of diagnosis with positive cd71 expression is considered when. Thus, cd71 expression level, in terms of mfi, is lower in leukemic erythroblasts. Evaluation of cd71 expression in acute leukemia in egyptian. We found the cd71 expression of the admixed normal erythroid population to be higher than all the leukemic blasts, which is in concordance with the earlier studies. Transferrin receptor cd71 mediates the uptake of transferriniron complexes and is highly expressed on the surface of cells of the erythroid lineage. Since each shrna was introduced sidebyside into control and. The hematopoiesis dataset and the differentiated myeloid cells were different data sets generated with different methods, i.
Cd71 expression was analyzed on the bone marrow blastic cells by flow cytometry. The cells were pelleted and resuspended in fresh megakaryocyte expansion media every 7 days. Twentyfive patients were diagnosed with aml, patients had ball, 8 patients had tall, and 2 patients had mpal b myeloid. In inversion 16 in addition to myeloid markers, they often express monocytic markers as cd14, cd64, cd4, cd11b, cd11c, cd36 and lysozyme. We studied 48 patients of acute leukemia, of which 25 were acute myeloid leukemia aml, were precursor bacute lymphoblastic leukemia ball, 8 were t. Cells were gently washed again and incubated on ice 30 min with 40. Cd71 transferrin receptor is a highly effective marker for the detection of cells of erythroid lineage in paraffin sections of bone marrow biopsy specimens.
Cd89 that is expressed on myeloid cells, including monocytemacrophages, neutrophils, eosinophils, and dendritic cells 3,4,5,6, the polymericig receptor polyigr that transports polymeric iga and. Il34 also induced increases in cd14 and cd68 expression, further supporting maturation toward monocytic character. The jak2v617f mutation is a promising candidate for molecularly targeted therapy in mpn. Tumorinduced generation of splenic erythroblastlike ter.
The objective of this study was to establish temporal changes in mirna expression in hl60 cells after treatment with tpa and overlay them with the existing database of immunophenotypic changes coincident with hl60. Significance of cd71 expression by flow cytometry in diagnosis of acute leukemia. Different expression of cd41 on human lymphoid and myeloid. Myelodysplastic syndromes mds are clonal disorders of the hematopoietic stem cell frequently presenting dysplasia in erythroid andor megakaryocytic lineage. Markers lthsc gmp cmp clp mep mpp b cells propre b cells myeloid dendritic cells plasmacytoid dendritic cells langerhans cells eosinophil neutrophil basophil macrophage. During this process, these early monocyte progenitors colonize various organs such as the brain, liver. Cd71 surface expression was measured on bone marrow blast cells. Markers lthsc gmp cmp clp mep mpp b cells propre b cells myeloid dendritic cells plasmacytoid dendritic cells langerhans cells eosinophil neutrophil basophil macrophage monocyte granulocyte mast cells precursor nk cells innate lymphoid progenitor double negative thymocyte double positive thymocyte gammadelta t cells nkt cells megakaryocyte. The mpn is serially transplantable and we demonstrate that the hematopoietic stem cell hsc compartment has the unique capacity for disease initiation but does not have a significant selective competitive advantage over wildtype hscs.
Purification strategy for isolating cells stained with cd34cd71 and hladr. Cd71expression was found to be significantly higher in acute myeloid leukemias than precursor b lymphoblastic leukemia p. Most acute myeloid leukemia progenitor cells with long. Flowcytometry not only can diagnose acute leukemia from peripheral bloodbone marrow, it can also detect leukemic cells in cerebrospinal fluid to detect central nervous system cns involvement, to start intracranial cns prophylaxis. Ltcic represent a rare population in the bone marrow, they have low forward light scatter, express cd34 and thy1, most lack. Monteiro,1 inserm e0225, bichat medical school, paris, france.
In the seven cases of acute erythroid leukemia ael, leukemic cells rarely expressed cd71, with the mean cd71 expression level significantly lower than that of acute megakaryocytic leukemia p cd71 is an integral membrane protein that mediates the uptake of transferriniron complexes. Differential expression and function of iga receptors. Role of cd71 in acute leukemia an immunophenotypic marker. Cd71 expression on the leukemic blasts was diversified.
Lower cd105 and cd71 expression for each population. Ssc low cells in a healthy stem cell donor a and in an mds patient b. In the absence of ongoing inflammatory and immune responses, dc constitutively patrol through the blood, peripheral tissues, lymph nodes, and secondary. The pattern of cd71 and ki67 expression in human t cells was investigated since an association between cd71 and ki67 was observed in mouse t cells at 24 h culture and beyond fig. Alloantigenactivated t regs demonstrated similar modulation of cd39, cd62l, cd38, cd24, cd69, and cd71 expression as observed on polyclonal stimulation, although the proportion of activation marker expressing cells was considerably higher than expected for markers such as cd39. The decreased proliferation of these myeloid cells also correlated with decreased cd71 transferrin receptor expression figure 1b, d.
Cd71 transferrin receptor american journal of clinical. American journal of respiratory cell and molecular biology. Cell surface markers of myeloid lineage, cd64 and cd86, remain constant while the levels of cd11b and cd71 decline with il34 treatment. Each day more than 10 12 cells are produced in the bone marrow from hematopoietic stem cells hscs. The majority of erythroblasts after transplantation with bcr ablexpressing c ebp cells exhibited high expression of cd71 fig. Differential expression and function of iga receptors cd89. Along with this, 5 normal bone marrow samples and 2 samples having increased number of hematogones were evaluated for cd71 expression. On days 14 and 28, the cells were analyzed for the expression of the megakaryocyte lineage markers cd41a and cd42b. Unique high and homogenous surface expression of the. The percentage of events in each gate is shown in the plot. The study included 34 acute myeloid leukemia aml and 64 acute lymphoblastic leukemia all newly diagnosed cases from mansoura oncology center.
Lsks mainly contain multipotent progenitors mpps that. Liu q1, wang m, hu y, xing h, chen x, zhang y, zhu p. Tpa treatment represses expression of cd4, cd117, cd95, cd64, and cd71 15. Prospective isolation of human erythroid lineagecommitted.
Absence of the transcription factor ccaat enhancer binding. Granulocytes, monocytes, macrophages, and dendritic cells dcs represent a subgroup of leukocytes, collectively called myeloid cells. Expression of cd71 by flow cytometry in acute leukemias. Its expression is very low on resting lymphocytes or thymocytes and it is lacking in mature red blood cells, polymorphonuclear cells and monocytes. Cd71 is known to be a proliferation marker theil, 1990. Significance of cd71 expression by flow cytometry in. Cd71 is a marker that has been usually used for identifying dysplasia in the erythroid series. Our approach provides a validated base panel that can be customized to incorporate additional cell identification markers such as those for defining t cells, nk cells, and b cells andor specific cell activation markers such as cd80, cd86, and cd71 expression on macrophages.
We are grateful to mark fleming, md, phd, for scientific contribution to this study and to alyson campbell for expert technical assistance. Cells were then cotransduced with shrna vectors for plzf target genes myc, id2, and dusp6 and plated in colony assays. Differentiation and expansion of hema topoietic precursor. In the seven cases of acute erythroid leukemia ael, leukemic cells rarely expressed cd71, with the mean cd71 expression level significantly lower than that of acute megakaryocytic leukemia p. Dendritic cells dc are the most effi tocompatibility. Cd71 is specific for the human transferrin receptor, 190 kilodaltons kda, 90kda reduced.
Expression of transferrin receptor on murine hematopoietic progenitors article pdf available in cellular immunology 831. Results plzf is expressed in human hematopoietic stem cells hscs and progenitors. Apr 17, 2003 the decreased proliferation of these myeloid cells also correlated with decreased cd71 transferrin receptor expression figure 1b, d. We included the circrna and the corresponding linear mrnas in this analysis that had at least 0. Human regulatory t cells with alloantigen specificity are. Differential expression and function of iga receptors cd89 and cd71 during maturation of dendritic cells benoit pasquier, yves lepelletier, ce. Moreover, flow cytometry findings suggest stag2 mutations may independently promote cell differentiation by increasing cd71 expression in a subset of cells.
The usefulness of cd71 expression by flow cytometry for. Plzf is a regulator of homeostatic and cytokineinduced. Hscs differentiate into various progenitor cells, which in turn generate many types of myeloid and lymphoid cells. Cd71 expression has been found to be significantly increased in rapidly proliferating cells.
Lymphoid antigens, such as cd2, cd5, cd7, cd19 and cd56, may be aberrantly expressed on myeloid progenitors and maturing myeloid cells in mds. During the embryonic development of mammalians, myelopoiesis occurs in a stepwise fashion that begins in the yolk sac and ends up in the bone marrow bm. Interestingly, besides transferrin, there are other cd71 ligands, which are linked to immunological processes, such as cd71 specific viruses and iga. In the seven cases of acute erythroid leukemia ael, leukemic cells rarely expressed cd71, with the mean cd71 expression level significantly lower than that of acute megakaryocytic leukemia p pdf available in cellular immunology 831. In recent years, it has been accepted that normal primitive longterm culture initiating cells ltcic7 can be distinguished and separated from more mature cells on the basis of their expressionlack of expression of specific cell surface markers. American journal of respiratory and critical care medicine. A cd71 and cd105 endoglin expression reveals phenotypic heterogeneity of myeloid progenitor cell compartments. During this process, these early monocyte progenitors colonize various organs. Translocation 8,21 is associated with expression of b lymphoid markers as cd19 or cd79a. Flow cytometry evaluation of erythroid and myeloid dysplasia. Although cd71 has been used for flow cytometric analysis, its usefulness in paraffinembedded bone marrow biopsy specimens has not been examined. Pdf range determination of antigen expression in myeloid. We report a jak2v617f knockin mouse myeloproliferative neoplasm mpn model resembling human polycythemia vera pv.
Determining the effect of jak2 inhibitors on the diseaseinitiating population requires a model in which the jak2v617f allele is. A common finding in mds is the aberrant expression of antigens on immature myeloid cells that are normally expressed on mature myeloid cells, such as cd11b andor cd15. Article tumorinduced generation of splenic erythroblastlike tercells promotes tumor progression yanmei han,1,9 qiuyan liu,1,9 jin hou,1,9 yan gu,1 yi zhang,1 zhubo chen,2 jia fan,3 weiping zhou,4 shuangjian qiu,3 yonghong zhang, 2,5tao dong, ning li,2,5 zhengping jiang,1 ha zhu,1 qian zhang,1 yuanwu ma,6 lianfeng zhang,6 qingqing wang,7 yizhi yu, 1nan. Correlation of transferrin receptor cd71 with ki67. Live cells were gated on the basis of pi staining a, and these cells were then gated on the basis of low side scatter b.
Cells were harvested, and mhc class ii and cd86 expression was assessed by flow cytometry. Most acute myeloid leukemia progenitor cells with longterm. Original article interleukin34 induces monocyticlike. Cd89 that is expressed on myeloid cells, including monocytemacrophages, neutrophils, eosinophils, and dendritic cells 3,4,5,6, the polymericig receptor polyigr that transports polymeric iga and igm across.
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